Lab 5 - miniPCR, DNA Restriction Enzyme Analysis, Evolution & Gel Electrophoresis


Cohort A - O'Bryant • Thursday January 26, 2017 Lab Assistants: labasst-benaduce.jpgCJ Beneduce lab_asst_hinton17_npreiss.jpgNick Preiss

Cohort B - Kennedy & TechBoston • Thursday February 2, 2017 Lab Assistants: labasst-zullo.jpgJoe Zullo labasst_hinton16_hderivera.jpgHeather DeRivera
Erica Mazaika
Cohort C - Boston Latin Academy Thursday February 9, 2017 Lab Assistants: lab_assnt-hawkins.jpgJessica Olivelab_asst_hinton17_aolive.jpgAndrew Olive
Heather de Rivera

Big Idea 3: Living systems store, retrieve, transmit and respond to information essential to life processes.

NOTE: The following lab was newly designed for Hinton16 students. The lab incorporates miniPCR technology to enable students to prepare their own DNA samples. Thank you to CJ Beneduce and David Chi for adapting and testing the experimental protocol. Hinton 17 students will use the new bluegel electrophoresis system to run gels.

Introduction



AP Biology Background Material

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Gel Electrophoresis Teacher Protocol Page T161
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Gel Electrophoresis Student Protocol Page S111




Student Experimental Protocol

Student teams will first use the miniPCR system to amplifiy DNA samples collected at a fictitious crime scene and then separate DNA fragments using the bluegel electrophoresis device. Each team will complete a set of worksheets given out during tutoring that cover the important theories and experiment steps required.
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miniPCR device

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blueGel Electrophoresis unit (NEW 2017)
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Version 1.4 (Updated 2/06/17)

Resources

Watch these videos before coming to lab.

DNA Replication


Polymerase Chain Reaction (PCR)


OPTIONAL - Enzyme Restriction

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Click to launch RFLP video