Lab 5 - DNA Analysis with miniPCR & Gel Electrophoresis

Cohort A - O'Bryant • Thursday January 31, 2019 • Lab Assistants labasst-benaduce.jpgCJ Beneduce
3 PM - TMEC Room 333

Cohort B - Kennedy & Tech Boston. • Thursday February 7, 2019 • Lab Assistantslabasst_hinton19_sganguli.jpgSangrag Ganguli
3 PM - TMEC Room 328

Cohort C - Boston Latin Academy • Thursday February 14, 2019 • Lab Assistantslabasst_hinton19_pbirla.jpgPakhi Birla
2:30 PM - TMEC Room 306

Big Idea 3: Living systems store, retrieve, transmit and respond to information essential to life processes.

NOTE: The following lab was newly designed for Hinton18 students. The lab incorporates miniPCR technology to enable students to prepare their own DNA samples. Thank you to CJ Beneduce and David Chi for adapting and testing the experimental protocol. Hinton 18 students will use the new bluegel electrophoresis system to run gels.


O' Bryant Big Ideas Form L 5&6
Kennedy and C.A.S.H.
Big Ideas Form L 5&6
Boston Latin Academy Big Ideas Form L 5&6


AP Biology Background Material

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Gel Electrophoresis Teacher Protocol Page T161
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Gel Electrophoresis Student Protocol Page S111

Student Experimental Protocol

Student teams will first use the miniPCR system to amplifiy DNA samples collected at a fictitious crime scene and then separate DNA fragments using the bluegel electrophoresis device. Each team will complete a set of worksheets given out during tutoring that cover the important theories and experiment steps required.
miniPCR device

blueGel Electrophoresis unit (NEW 2017)

Lab Protocol
Version 1.4 (Updated 1/31/18)


Watch these videos before coming to lab.

DNA Replication

Polymerase Chain Reaction (PCR)

OPTIONAL - Enzyme Restriction

Click to launch RFLP video